Journal:

Article Title: The effect of mutated mitochondrial ribosomal proteins S16 and S22 on the assembly of the small and large ribosomal subunits in human mitochondria

doi: 10.1016/j.mito.2008.04.004

Figure Lengend Snippet: Identification of human mitochondrial ribosomal proteins in control and MRPS16 patient cells. (A) The levels of patient mitochondrial ribosomal proteins MRPS16, MRPS2, MRPS11, MRPL13 and MRPL15 from healthy control and MRPS16 cells were determined by Western blotting using antibodies against the specified protein and detected by ECL. The background of the blots varied due the type of the film used, the exposure time and the number of times the membrane was re-probed. In each case, control and patient mitochondria were examined under the same conditions. The antibody used is indicated as the probe above the given lanes. (B) Quantification of the amount of the indicated protein present in fibroblast mitochondria from the MRPS16 patient compared to the amount present in control cells. Relative band intensity was determined using the UNSCAN-IT software.

Article Snippet: The cDNAs encoding human mitochondrial ribosomal proteins MRPS2 (ATCC number MGC-16952), MRPS11 (ATCC number MGC-40231), MRPL13 (ATCC number MGC-33899) and MRPL15 (ATCC MGC-4898) were obtained from American Type Culture Collection (ATCC).

Techniques: Control, Western Blot, Membrane, Software

Journal:

Article Title: The effect of mutated mitochondrial ribosomal proteins S16 and S22 on the assembly of the small and large ribosomal subunits in human mitochondria

doi: 10.1016/j.mito.2008.04.004

Figure Lengend Snippet: Identification of human mitochondrial ribosomal proteins of control and MRPS22 patient cells. (A) The levels of mitochondrial ribosomal proteins MRPS16, MRPS2, MRPS11, MRPL13 and MRPL15 were examined in mitochondria derived from control and MRPS22 cells using Western blotting and the indicated antibody as a probe. The backgrounds on the blots depended on the type of film used, and the number of times the membranes were stripped for reprobing. (B) Quantification of the amount of the indicated protein present in fibroblast mitochondria from the MRPS16 patient compared to the amount present in control cells. Relative band intensities were determined using the SCAN-IT software.

Article Snippet: The cDNAs encoding human mitochondrial ribosomal proteins MRPS2 (ATCC number MGC-16952), MRPS11 (ATCC number MGC-40231), MRPL13 (ATCC number MGC-33899) and MRPL15 (ATCC MGC-4898) were obtained from American Type Culture Collection (ATCC).

Techniques: Control, Derivative Assay, Western Blot, Software